› 2vq5

X-ray diffraction
2.09Å resolution

X-ray structure of Norcoclaurine synthase from Thalictrum flavum in complex with dopamine and hydroxybenzaldehyde

Released:
DOI: 10.2210/pdb2vq5/pdb
PDB entry 2vq5 coloured by chain, front view.
PDB entry 2vq5 coloured by chain, side view.
PDB entry 2vq5 coloured by chain, top view.
Source organism: Thalictrum flavum
Primary publication:
Structural basis of enzymatic (S)-norcoclaurine biosynthesis.
Ilari A, Franceschini S, Bonamore A, Arenghi F, Botta B, Macone A, Pasquo A, Bellucci L, Boffi A
J Biol Chem 284 897-904 (2009)
PMID: 19004827

Function and Biology Details

Reaction catalysed: 
Dopamine + 4-hydroxyphenylacetaldehyde = (S)-norcoclaurine + H(2)O
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-norcoclaurine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.61 KDa
Source organism: Thalictrum flavum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q67A25 (Residues: 16-210; Coverage: 100%)
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

4 bound ligands:
Ligand ACT 2 x ACT
Ligand CL 5 x CL
Ligand HBA 2 x HBA
Ligand LDP 1 x LDP
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 86.474Å b: 86.474Å c: 117.977Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.266
Expression system: Escherichia coli BL21(DE3)

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Citations

23 review citations

The Pictet-Spengler reaction in nature and in organic chemistry.
Stöckigt et al. (2011)
22 more

10 mentions without citation

Structures of two Arabidopsis thaliana major latex proteins represent novel helix-grip folds.
Lytle et al. (2009)
9 more