› 2vdc

Electron Microscopy
9.5Å resolution

THE 9.5 A RESOLUTION STRUCTURE OF GLUTAMATE SYNTHASE FROM CRYO-ELECTRON MICROSCOPY AND ITS OLIGOMERIZATION BEHAVIOR IN SOLUTION: FUNCTIONAL IMPLICATIONS.

Released:
DOI: 10.2210/pdb2vdc/pdb
PDB entry 2vdc coloured by chain, front view.
PDB entry 2vdc coloured by chain, side view.
PDB entry 2vdc coloured by chain, top view.
Source organism: Azospirillum brasilense
Primary publication:
The subnanometer resolution structure of the glutamate synthase 1.2-MDa hexamer by cryoelectron microscopy and its oligomerization behavior in solution: functional implications.
Cottevieille M, Larquet E, Jonic S, Petoukhov MV, Caprini G, Paravisi S, Svergun DI, Vanoni MA, Boisset N
J Biol Chem 283 8237-49 (2008)
PMID: 18199747
Related structures: EMD-1440

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamate + NH(3) = L-glutamine + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170243 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutamate synthase [NADPH] large chain Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 1472 amino acids
Theoretical weight: 161.62 KDa
Source organism: Azospirillum brasilense
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05755 (Residues: 37-1508; Coverage: 97%)
Gene name: gltB
Sequence domains:
Glutamate synthase [NADPH] small chain Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 456 amino acids
Theoretical weight: 49.37 KDa
Source organism: Azospirillum brasilense
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05756 (Residues: 27-482; Coverage: 95%)
Gene name: gltD
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 6 x FAD

Cofactor: Ligand FMN 6 x FMN
4 bound ligands:
Ligand OMT 6 x OMT
Ligand AKG 6 x AKG
Ligand F3S 6 x F3S
Ligand SF4 12 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.5Å
Relevant EMDB volumes: EMD-1440
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Brucella, nitrogen and virulence.
Ronneau et al. (2016)
2 more

4 mentions without citation

On the Natural History of Flavin-Based Electron Bifurcation.
Baymann et al. (2018)
3 more