2uw5 Summary

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STRUCTURE OF PKA-PKB CHIMERA COMPLEXED WITH (R)-2-(4-CHLORO-PHENYL)-2-(4-1H-PYRAZOL-4-YL)-PHENYL)-ETHYLAMINE

The structure was published by Saxty, G., Woodhead, S.J., Berdini, V., et al., Downham, R., Garrett, M.D., and Carr, R.A., in 2007 in a paper entitled "Identification of Inhibitors of Protein Kinase B Using Fragment-Based Lead Discovery" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.14 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT and CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT P00517 (2-351) (KAPCA_BOVIN)search Bos taurussearch 96% 351 95%
I CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA P61925 (6-25) (IPKA_HUMAN)search Homo sapienssearch 100% 20 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00517 (2 - 351) CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT Bos taurus
P61925 (6 - 25) CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA HOMO SAPIENS

Chain Structural classification (CATH) Sequence family (Pfam)
A (P00517) Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
I (P61925) PF02827: cAMP-dependent protein kinase inhibitorsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P00517) protein kinase activitysearch protein serine/threonine kinase activitysearch protein bindingsearch transferase activity, transferring phosphorus-containing groupssearch cAMP-dependent protein kinase activitysearch protein kinase A regulatory subunit bindingsearch protein serine/threonine/tyrosine kinase activitysearch protein kinase bindingsearch ubiquitin protein ligase bindingsearch ATP bindingsearch transferase activitysearch kinase activitysearch nucleotide bindingsearch regulation of synaptic transmissionsearch peptidyl-serine phosphorylationsearch regulation of protein processingsearch protein autophosphorylationsearch positive regulation of cell cycle arrestsearch sperm capacitationsearch regulation of osteoblast differentiationsearch mesoderm formationsearch positive regulation of protein export from nucleussearch regulation of proteasomal protein catabolic processsearch phosphorylationsearch regulation of tight junction assemblysearch cellular response to glucose stimulussearch peptidyl-threonine phosphorylationsearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch protein phosphorylationsearch neural tube closuresearch cellular response to parathyroid hormone stimulussearch AMP-activated protein kinase complexsearch nucleussearch cytoplasmsearch neuromuscular junctionsearch plasma membranesearch mitochondrionsearch extracellular vesicular exosomesearch membranesearch sperm midpiecesearch centrosomesearch ciliary basesearch
I (P61925) cAMP-dependent protein kinase inhibitor activitysearch negative regulation of protein kinase activitysearch

Chain InterPro annotation
A Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
I cAMP-dependent protein kinase inhibitorsearch