› 2qla

X-ray diffraction
2.9Å resolution

Crystal Structure of a 16-Helix Bundle Architecture Produced by the Zinc-Mediated Self Assembly of Four Cytochrome cb562 Molecules

Released:
DOI: 10.2210/pdb2qla/pdb
PDB entry 2qla coloured by chain, front view.
PDB entry 2qla coloured by chain, side view.
PDB entry 2qla coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Controlling protein-protein interactions through metal coordination: assembly of a 16-helix bundle protein.
Salgado EN, Faraone-Mennella J, Tezcan FA
J Am Chem Soc 129 13374-5 (2007)
PMID: 17929927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P21212
Unit cell:
a: 88.984Å b: 66.659Å c: 68.901Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.295
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Design of functional metalloproteins.
Lu et al. (2009)
18 more

3 mentions without citation

Controlling protein-protein interactions through metal coordination: assembly of a 16-helix bundle protein.
Salgado et al. (2007)
2 more