› 2f7t

X-ray diffraction
2.25Å resolution

Crystal structure of the catalytic domain of Mos1 mariner transposase

Released:
DOI: 10.2210/pdb2f7t/pdb
PDB entry 2f7t coloured by chain, front view.
PDB entry 2f7t coloured by chain, side view.
PDB entry 2f7t coloured by chain, top view.
Source organism: Drosophila mauritiana
Primary publication:
Mechanism of Mos1 transposition: insights from structural analysis.
Richardson JM, Dawson A, O'Hagan N, Taylor P, Finnegan DJ, Walkinshaw MD
EMBO J 25 1324-34 (2006)
PMID: 16511570

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mariner Mos1 transposase Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 27.18 KDa
Source organism: Drosophila mauritiana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7JQ07 (Residues: 119-345; Coverage: 66%)
Gene name: mariner\T
Sequence domains: Transposase (partial DDE domain)
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 44.243Å b: 44.243Å c: 206.047Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.189 0.277
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Mechanisms of DNA Transposition.
Hickman et al. (2015)
8 more

11 mentions without citation

Integrating prokaryotes and eukaryotes: DNA transposases in light of structure.
Hickman et al. (2010)
10 more