Function and Biology Details
Biochemical function:
- not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150517 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease
Molecule details ›
Chains: A, D
Length: 82 amino acids
Theoretical weight: 9.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Vps23 core domain
Structure domains: Helix Hairpins
Length: 82 amino acids
Theoretical weight: 9.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P25604 (Residues: 305-385; Coverage: 21%)
Sequence domains: Vps23 core domain
Structure domains: Helix Hairpins
Vacuolar protein sorting-associated protein 28
Molecule details ›
Chains: B, E
Length: 155 amino acids
Theoretical weight: 17.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: VPS28 protein
Structure domains: de novo design (two linked rop proteins)
Length: 155 amino acids
Theoretical weight: 17.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q02767 (Residues: 1-147; Coverage: 61%)
Sequence domains: VPS28 protein
Structure domains: de novo design (two linked rop proteins)
Protein SRN2
Molecule details ›
Chains: C, F
Length: 85 amino acids
Theoretical weight: 10.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Modifier of rudimentary (Mod(r)) protein
Structure domains: Helix hairpin bin
Length: 85 amino acids
Theoretical weight: 10.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q99176 (Residues: 130-213; Coverage: 39%)
Sequence domains: Modifier of rudimentary (Mod(r)) protein
Structure domains: Helix hairpin bin
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-4
Spacegroup:
P61
Expression system: Escherichia coli BL21(DE3)
