› 2b1d

X-ray diffraction
2.5Å resolution

5'-D(*GP*CP*AP*GP*AP*CP*GP*TP*CP*TP*GP*C)-3' Methionine Repressor binding site

Released:
DOI: 10.2210/pdb2b1d/pdb
PDB entry 2b1d coloured by chain, front view.
PDB entry 2b1d coloured by chain, side view.
PDB entry 2b1d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Signatures of protein-DNA recognition in free DNA binding sites.
Locasale JW, Napoli AA, Chen S, Berman HM, Lawson CL
J Mol Biol 386 1054-65 (2009)
PMID: 19244617

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115102 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*GP*CP*AP*GP*AP*CP*GP*TP*CP*TP*GP*C)-3' Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NCO 3 x NCO
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P1
Unit cell:
a: 39.98Å b: 40.346Å c: 40.255Å
α: 82.15° β: 69.13° γ: 81.44°
R-values:
R R work R free
0.239 0.235 0.284
Expression system: Not provided

Quick links

Citations

2 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
1 more

3 mentions without citation

Signatures of protein-DNA recognition in free DNA binding sites.
Locasale et al. (2009)
2 more