› 2uuz

X-ray diffraction
2.3Å resolution

Orthorhombic crystal form of GamS from bacteriophage lambda.

Released:
DOI: 10.2210/pdb2uuz/pdb
PDB entry 2uuz coloured by chain, front view.
PDB entry 2uuz coloured by chain, side view.
PDB entry 2uuz coloured by chain, top view.
Source organism: Escherichia virus Lambda
Primary publication:
The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition.
Court R, Cook N, Saikrishnan K, Wigley D
J Mol Biol 371 25-33 (2007)
PMID: 17544443

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Host-nuclease inhibitor protein gam Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.73 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03702 (Residues: 40-138; Coverage: 72%)
Gene names: gam, gamma
Sequence domains: Host-nuclease inhibitor protein Gam
Structure domains: Host-nuclease inhibitor protein Gam

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 142.358Å b: 39.147Å c: 44.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.279
Expression system: Escherichia coli

Quick links

Citations

10 review citations

RecBCD enzyme and the repair of double-stranded DNA breaks.
Dillingham et al. (2008)
9 more

4 mentions without citation

The protein interaction map of bacteriophage lambda.
Rajagopala et al. (2011)
3 more