› 2a0i

X-ray diffraction
2.72Å resolution

F Factor TraI Relaxase Domain bound to F oriT Single-stranded DNA

Released:
DOI: 10.2210/pdb2a0i/pdb
PDB entry 2a0i coloured by chain, front view.
PDB entry 2a0i coloured by chain, side view.
PDB entry 2a0i coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Inter- and intramolecular determinants of the specificity of single-stranded DNA binding and cleavage by the F factor relaxase.
Larkin C, Datta S, Harley MJ, Anderson BJ, Ebie A, Hargreaves V, Schildbach JF
Structure 13 1533-44 (2005)
PMID: 16216584

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115057 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Multifunctional conjugation protein TraI Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14565 (Residues: 1-330; Coverage: 19%)
Gene names: ECOK12F104, traI
Sequence domains: TrwC relaxase
F plasmid single-stranded oriT DNA Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.93 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P62
Unit cell:
a: 77.515Å b: 77.515Å c: 114.369Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.215 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

The diversity of conjugative relaxases and its application in plasmid classification.
Garcillán-Barcia et al. (2009)
9 more

12 mentions without citation

The structure of the minimal relaxase domain of MobA at 2.1 A resolution.
Monzingo et al. (2007)
11 more