› 252d

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING

Released:
DOI: 10.2210/pdb252d/pdb
PDB entry 252d coloured by chain, front view.
PDB entry 252d coloured by chain, side view.
PDB entry 252d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Sequence-dependent crossed helix packing in the crystal structure of a B-DNA decamer yields a detailed model for the Holliday junction.
Wood AA, Nunn CM, Trent JO, Neidle S
J Mol Biol 269 827-41 (1997)
PMID: 9223644

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115014 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*AP*AP*TP*TP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: C2
Unit cell:
a: 63.63Å b: 24.7Å c: 38.29Å
α: 90° β: 110.27° γ: 90°
R-values:
R R work R free
0.177 0.177 not available
Expression system: Not provided

Quick links

Citations

2 review citations

The crystal structures of DNA Holliday junctions.
Ho et al. (2001)
1 more

3 mentions without citation

The inherent properties of DNA four-way junctions: comparing the crystal structures of holliday junctions.
Eichman et al. (2002)
2 more