› 1w7z

X-ray diffraction
1.67Å resolution

Crystal structure of the free (uncomplexed) Ecballium elaterium trypsin inhibitor (EETI-II)

Released:
DOI: 10.2210/pdb1w7z/pdb
PDB entry 1w7z coloured by chain, front view.
PDB entry 1w7z coloured by chain, side view.
PDB entry 1w7z coloured by chain, top view.
Source organism: Ecballium elaterium
Primary publication:
Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.
Krätzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I
Acta Crystallogr D Biol Crystallogr 61 1255-62 (2005)
PMID: 16131759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-146094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin inhibitor 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 31 amino acids
Theoretical weight: 3.14 KDa
Source organism: Ecballium elaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P12071 (Residues: 1-30; Coverage: 100%)
Sequence domains: Squash family serine protease inhibitor

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand FMT 3 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 71.16Å b: 82.34Å c: 62.93Å
α: 90° β: 118.8° γ: 90°
R-values:
R R work R free
0.197 0.194 0.235
Expression system: Escherichia coli

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Citations

3 review citations

Advances, interactions, and future developments in the CNS, Phenix, and Rosetta structural biology software systems.
Adams et al. (2013)
2 more

6 mentions without citation

Small-Format Drug Conjugates: A Viable Alternative to ADCs for Solid Tumours?
Deonarain et al. (2018)
5 more