› 1qsx

Solution NMR

SOLUTION NMR STRUCTURE OF THE 2:1 HOECHST 33258-D(CTTTTGCAAAAG)2 COMPLEX

Released:
DOI: 10.2210/pdb1qsx/pdb
PDB entry 1qsx coloured by chain, front view.
PDB entry 1qsx coloured by chain, side view.
PDB entry 1qsx coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Sequence-dependent variation in DNA minor groove width dictates orientational preference of Hoechst 33258 in A-tract recognition: solution NMR structure of the 2:1 complex with d(CTTTTGCAAAAG)(2).
Gavathiotis E, Sharman GJ, Searle MS
Nucleic Acids Res 28 728-35 (2000)
PMID: 10637324

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114470 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(CP*TP*TP*TP*TP*GP*CP*AP*AP*AP*AP*G)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 19 x NA
Ligand HT 2 x HT
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: 1NS RESTRAINED MOLECULAR DYNAMICS
Expression system: Not provided

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Citations

3 review citations

Thermodynamics of drug-DNA interactions.
Haq I. (2002)
2 more

2 mentions without citation

Sequence-dependent variation in DNA minor groove width dictates orientational preference of Hoechst 33258 in A-tract recognition: solution NMR structure of the 2:1 complex with d(CTTTTGCAAAAG)(2).
Gavathiotis et al. (2000)
1 more