› 1lli

X-ray diffraction
2.1Å resolution

THE CRYSTAL STRUCTURE OF A MUTANT PROTEIN WITH ALTERED BUT IMPROVED HYDROPHOBIC CORE PACKING

Released:
DOI: 10.2210/pdb1lli/pdb
PDB entry 1lli coloured by chain, front view.
PDB entry 1lli coloured by chain, side view.
PDB entry 1lli coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
The crystal structure of a mutant protein with altered but improved hydrophobic core packing.
Lim WA, Hodel A, Sauer RT, Richards FM
Proc Natl Acad Sci U S A 91 423-7 (1994)
PMID: 8278404

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114140 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Repressor protein cI Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 10.15 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03034 (Residues: 2-93; Coverage: 39%)
Gene names: cI, lambdap88
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*AP*TP*AP*CP*CP*AP*CP*TP*GP*GP*CP*GP*GP*TP*GP*A P*TP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
DNA (5'-D(*TP*AP*TP*AP*TP*CP*AP*CP*CP*GP*CP*CP*AP*GP*TP*GP*G P*TP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 37.15Å b: 68.9Å c: 56.79Å
α: 90° β: 92.2° γ: 90°
R-values:
R R work R free
0.196 0.196 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
15 more

6 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
5 more