› 1l8f

X-ray diffraction
1.8Å resolution

Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A

Released:
DOI: 10.2210/pdb1l8f/pdb
PDB entry 1l8f coloured by chain, front view.
PDB entry 1l8f coloured by chain, side view.
PDB entry 1l8f coloured by chain, top view.
Source organism: Melanocarpus albomyces
Primary publication:
Structure of 20K endoglucanase from Melanocarpus albomyces at 1.8 A resolution.
Valjakka J, Rouvinen J
Acta Crystallogr D Biol Crystallogr 59 765-8 (2003)
PMID: 12657806

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulase Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 22.23 KDa
Source organism: Melanocarpus albomyces
UniProt:
  • Canonical: Q8J0K8 (Residues: 22-228; Coverage: 97%)
Gene name: cel45A
Sequence domains: Glycosyl hydrolase family 45
Structure domains: RlpA-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 47.44Å b: 47.44Å c: 156.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.257

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Citations

4 citation in other articles

Functional Characterization and Low-Resolution Structure of an Endoglucanase Cel45A from the Filamentous Fungus Neurospora crassa OR74A: Thermostable Enzyme with High Activity Toward Lichenan and β-Glucan.
Kadowaki et al. (2015)
3 more