› 1jrz

X-ray diffraction
2Å resolution

Crystal structure of Arg402Tyr mutant flavocytochrome c3 from Shewanella frigidimarina

Released:
DOI: 10.2210/pdb1jrz/pdb
PDB entry 1jrz coloured by chain, front view.
PDB entry 1jrz coloured by chain, side view.
PDB entry 1jrz coloured by chain, top view.
Source organism: Shewanella frigidimarina
Primary publication:
Kinetic and crystallographic analysis of the key active site acid/base arginine in a soluble fumarate reductase.
Mowat CG, Moysey R, Miles CS, Leys D, Doherty MK, Taylor P, Walkinshaw MD, Reid GA, Chapman SK
Biochemistry 40 12292-8 (2001)
PMID: 11591148

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate reductase (cytochrome) Chains: A, B
Molecule details ›
Chains: A, B
Length: 571 amino acids
Theoretical weight: 60.63 KDa
Source organism: Shewanella frigidimarina
Expression system: Shewanella frigidimarina NCIMB 400
UniProt:
  • Canonical: P0C278 (Residues: 1-571; Coverage: 100%)
Gene names: fcc3, fccA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand HEM 8 x HEM
2 bound ligands:
Ligand NA 2 x NA
Ligand FUM 2 x FUM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 78.072Å b: 87.906Å c: 90.163Å
α: 90° β: 105.03° γ: 90°
R-values:
R R work R free
0.175 0.176 0.255
Expression system: Shewanella frigidimarina NCIMB 400

Quick links

Citations

5 review citations

Molecular recognition of oxoanions based on guanidinium receptors.
Blondeau et al. (2007)
4 more

1 mention without citation

Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations.
Steinkellner et al. (2014)