› 1j1w

X-ray diffraction
3.2Å resolution

Crystal Structure Of The Monomeric Isocitrate Dehydrogenase In Complex With NADP+

Released:
DOI: 10.2210/pdb1j1w/pdb
PDB entry 1j1w coloured by chain, front view.
PDB entry 1j1w coloured by chain, side view.
PDB entry 1j1w coloured by chain, top view.
Source organism: Azotobacter vinelandii
Primary publication:
Crystal structure of the monomeric isocitrate dehydrogenase in the presence of NADP+: insight into the cofactor recognition, catalysis, and evolution.
Yasutake Y, Watanabe S, Yao M, Takada Y, Fukunaga N, Tanaka I
J Biol Chem 278 36897-904 (2003)
PMID: 12855708

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 741 amino acids
Theoretical weight: 80.51 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P16100 (Residues: 1-741; Coverage: 100%)
Gene name: icd
Sequence domains: Monomeric isocitrate dehydrogenase

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 113.496Å b: 110.407Å c: 133.698Å
α: 90° β: 90.156° γ: 90°
R-values:
R R work R free
0.26 0.26 0.297

Quick links

Citations

23 citation in other articles

Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability.
Fedøy et al. (2007)
22 more

2 mentions without citation

Novel type II and monomeric NAD+ specific isocitrate dehydrogenases: phylogenetic affinity, enzymatic characterization, and evolutionary implication.
Wang et al. (2015)
1 more