› 1hv8

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII

Released:
DOI: 10.2210/pdb1hv8/pdb
PDB entry 1hv8 coloured by chain, front view.
PDB entry 1hv8 coloured by chain, side view.
PDB entry 1hv8 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii.
Story RM, Li H, Abelson JN
Proc Natl Acad Sci U S A 98 1465-70 (2001)
PMID: 11171974

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent RNA helicase MJ0669 Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 42.5 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58083 (Residues: 1-367; Coverage: 100%)
Gene name: MJ0669
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 109.132Å b: 131.45Å c: 131.829Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.27 0.316
Expression system: Escherichia coli

Quick links

Citations

26 review citations

The DEAD-box protein family of RNA helicases.
Cordin et al. (2006)
25 more

24 mentions without citation

Structural basis for the enhancement of eIF4A helicase activity by eIF4G.
Oberer et al. (2005)
23 more