› 1f89

X-ray diffraction
2.4Å resolution

Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily

Released:
DOI: 10.2210/pdb1f89/pdb
PDB entry 1f89 coloured by chain, front view.
PDB entry 1f89 coloured by chain, side view.
PDB entry 1f89 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of a putative CN hydrolase from yeast.
Kumaran D, Eswaramoorthy S, Gerchman SE, Kycia H, Studier FW, Swaminathan S
Proteins 52 283-91 (2003)
PMID: 12833551

Function and Biology Details

Reaction catalysed: 
A monoamide of a dicarboxylate + H(2)O = a dicarboxylate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Omega-amidase NIT3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 32.59 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P49954 (Residues: 1-291; Coverage: 100%)
Gene names: L9638.5, NIT3, YLR351C
Sequence domains: Carbon-nitrogen hydrolase
Structure domains: Carbon-nitrogen hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 73.523Å b: 53.492Å c: 80.985Å
α: 90° β: 112.43° γ: 90°
R-values:
R R work R free
0.229 0.229 0.258
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Primary or secondary? Versatile nitrilases in plant metabolism.
Piotrowski M. (2008)
2 more

13 mentions without citation

Structural genomics: a pipeline for providing structures for the biologist.
Chance et al. (2002)
12 more