› 1wxj

X-ray diffraction
1.7Å resolution

Crystal Structure Of Tryptophan Synthase A-Subunit with Indole-3-propanol phosphate From Thermus Thermophilus Hb8

Released:
DOI: 10.2210/pdb1wxj/pdb
PDB entry 1wxj coloured by chain, front view.
PDB entry 1wxj coloured by chain, side view.
PDB entry 1wxj coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Asada Y, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 28.98 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16608 (Residues: 1-270; Coverage: 100%)
Gene names: TT_C0729, trpA
Sequence domains: Tryptophan synthase alpha chain
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand IPL 1 x IPL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 42.631Å b: 76.395Å c: 42.721Å
α: 90° β: 115.99° γ: 90°
R-values:
R R work R free
0.19 0.189 0.21
Expression system: Escherichia coli BL21(DE3)

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