› 1or4

X-ray diffraction
2.15Å resolution

Crystal Structure of HemAT sensor domain from B.subtilis in the cyano-liganded form

Released:
DOI: 10.2210/pdb1or4/pdb
PDB entry 1or4 coloured by chain, front view.
PDB entry 1or4 coloured by chain, side view.
PDB entry 1or4 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of the oxygen sensor in Bacillus subtilis: signal transduction of chemotaxis by control of symmetry.
Zhang W, Phillips GN
Structure 11 1097-110 (2003)
PMID: 12962628

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126601 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme-based aerotactic transducer HemAT Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.84 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O07621 (Residues: 1-178; Coverage: 41%)
Gene names: BSU10380, hemAT, yhfV
Sequence domains: Protoglobin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand CYN 2 x CYN
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C, null
Spacegroup: P212121
Unit cell:
a: 49.994Å b: 80.117Å c: 85.944Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Diversity in chemotaxis mechanisms among the bacteria and archaea.
Szurmant et al. (2004)
9 more

13 mentions without citation

Sensory Repertoire of Bacterial Chemoreceptors.
Ortega et al. (2017)
12 more