1ku3

X-ray diffraction
1.8Å resolution

Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment, Region 4

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-190227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase sigma factor SigA Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.74 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EZJ8 (Residues: 366-438; Coverage: 17%)
Gene name: sigA
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 39.804Å b: 39.804Å c: 66.807Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.252
Expression system: Escherichia coli BL21(DE3)