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Workflow for eDNA metabarcoding

Environmental projects that assess biodiversity through eDNA metabarcoding involve several key steps as shown in the following flowchart (Figure 2).

Flowchart illustrating the steps in DNA analysis: sampling, DNA extraction, sequencing, and bioinformatics. Each step is connected as arrows heads to show the progression from collecting samples to analysing DNA data.
Figure 2 A flowchart showing key steps required in any eDNA study.

Each of these steps requires the careful selection of suitable methods and technologies. In the following pages you will identify the factors to consider when making these decisions for your project.