M-CSA is a database of enzyme reaction mechanisms. It provides annotation on the protein, catalytic residues, cofactors, and the reaction mechanisms of hundreds of enzymes. There are two kinds of entries in M-CSA. 'Detailed mechanism' entries are more complete and show the individual chemical steps of the mechanism as schemes with electron flow arrows. 'Catalytic Site' entries annotate the catalytic residues necessary for the reaction, but do not show the mechanism.

Glutamate racemase

example mechanism

Biological species: Aquifex pyrophilus (Bacteria) Uniprot
Sequence: P56868 UniProt
PDB: 1b73 PDBe PDBsum 1b73
Catalytic CATH domains: 3.40.50.1860 CATHdb

Glutamate racemase is responsible for the synthesis of D-glutamate, an essential building block of peptidoglycan, found in bacterial cell walls where it provides structural integrity. Due to its uniqueness to bacteria, peptidoglycan, and enzymes involved in its biosynthesis, are targets for designing new antibacterial drugs. Peptidoglycan is formed from a repeating unit of a disaccharide, N-acetylglucosamine and N-acetylmuramic acid to which a small group of amino acids (L-alanine, D-alaline, D-glutamate and either lysine or diaminopimelic acid) are covalently attached. The presence of D-amino acids protects the cell wall from proteases which can only recognise the L-isomer.

Features

Latest Statistics

As of 18th November 2018, M-CSA contains 964 hand-curated entries, 684 of them with detailed mechanistic description. There are 1409 UniProt sequences associated with these entries, 15566 pdb structures, 1001 CATH domains, and 852 EC numbers.

Last updates

New Website
We have merged CSA (Catalytic Site Atlas) and MACiE (Mechanism Annotation and Classification in Enzymes) into the same database and website. The new website facilitates the data entry process and supports different mechanism proposals, among others improvements. It is powered by the Python Django web framework and a PostgreSQL database.

To cite M-CSA

(see complete list of related papers)
  • Ribeiro AJM et al. (2017), Nucleic Acids Res, , -. Mechanism and Catalytic Site Atlas (M-CSA): a database of enzyme reaction mechanisms and active sites. DOI:10.1093/nar/gkx1012.