PDBsum entry 8rjo

Signaling protein

PDB id: 8rjo

Contents

Protein chains

490 a.a.

Ligands

EL5 ×2

SO4 ×4

Waters ×203

PDB id:

8rjo

Name:

Signaling protein

Title:

Serial femtosecond x-ray structure of a fluorescence optimized bathy phytochrome pairfp2 derived from wild-type agp2 in i1 intermediate state.

Structure:

Histidine kinase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Agrobacterium fabrum str. C58. Organism_taxid: 176299. Gene: atu2165. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.54Å R-factor: 0.207 R-free: 0.236

Authors:

L.Sauthof,A.Schmidt,M.Szczepek,A.S.Brewster,J.F.Kern,P.Scheerer

Key ref:

L.Sauthof et al. Serial-Femtosecond crystallography reveals how a phyt variant couples chromophore and protein structural ch. Sci adv, . PubMed id: 40435264

Date:

21-Dec-23 Release date: 14-May-25

Protein chains

A9CI81  (A9CI81_AGRFC) -  histidine kinase from Agrobacterium fabrum (strain C58 / ATCC 33970)

Seq: 850 a.a.

Struc: 490 a.a.*

* PDB and UniProt seqs differ at 23 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.13.3 - histidine kinase.
• Reaction: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site