PDBsum entry 8dcr

Signaling protein

PDB id: 8dcr

Contents

Protein chains

338 a.a.

130 a.a.

200 a.a.

57 a.a.

275 a.a.

Ligands

Y00

PDB id:

8dcr

Name:

Signaling protein

Title:

Cryo-em structure of dobutamine-bound beta1-adrenergic receptor in complex with heterotrimeric gs-protein

Structure:

Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes. Nanobody 35. Chain: n. Engineered: yes. Guanine nucleotide-binding protein g(s) subunit alpha

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Gene: gnb1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Lama glama. Organism_taxid: 9844. Expressed in: escherichia coli.

Authors:

M.Su,N.Paknejad,R.K.Hite,X.Y.Huang

Key ref:

M.Su et al. (2022). Structures of beta 1 -Adrenergic receptor in complex and ligands of different efficacies.. Nat commun, 13, 4095. PubMed id: 35835792

Date:

17-Jun-22 Release date: 27-Jul-22

Protein chain

P62871  (GBB1_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Bos taurus

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

No UniProt id for this chain

Struc: 130 a.a.

Protein chain

P04896  (GNAS2_BOVIN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Bos taurus

Seq: 394 a.a.

Struc: 200 a.a.*

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 57 a.a.

Protein chain

P00720  (ENLYS_BPT4) -  Endolysin from Enterobacteria phage T4

Seq: 164 a.a.

Struc: 275 a.a.*

Protein chain

P07700  (ADRB1_MELGA) -  Beta-1 adrenergic receptor from Meleagris gallopavo

Seq: 483 a.a.

Struc: 275 a.a.*

* PDB and UniProt seqs differ at 119 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 2: Chain R: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.