PDBsum entry 7mt8

Membrane protein, signaling protein

PDB id: 7mt8

Contents

Protein chains

346 a.a.

324 a.a.

Ligands

SGV

RET

PDB id:

7mt8

Name:

Membrane protein, signaling protein

Title:

Rhodopsin kinase (grk1)-s5e/s488e/t489e in complex with rhodopsin

Structure:

Rhodopsin kinase grk1. Chain: g. Synonym: rk,g protein-coupled receptor kinase 1. Engineered: yes. Mutation: yes. Rhodopsin. Chain: r

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Gene: grk1, rhok. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Organism_taxid: 9913

Ensemble:

6 models

Authors:

Q.Chen,C.-L.Chen,J.J.G.Tesmer

Key ref:

Q.Chen et al. (2021). Structures of rhodopsin in complex with g-Protein-Cou receptor kinase 1.. Nature, 595, 600. PubMed id: 34262173 DOI: 10.1038/S41586-021-03721-X

Date:

13-May-21 Release date: 07-Jul-21

Protein chain

P28327  (RK_BOVIN) -  Rhodopsin kinase GRK1 from Bos taurus

Seq: 561 a.a.

Struc: 346 a.a.*

Protein chain

P02699  (OPSD_BOVIN) -  Rhodopsin from Bos taurus

Seq: 348 a.a.

Struc: 324 a.a.

* PDB and UniProt seqs differ at 20 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain G: E.C.2.7.11.14 - rhodopsin kinase.
• Reaction:
  1. L-seryl-[rhodopsin] + ATP = O-phospho-L-seryl-[rhodopsin] + ADP + H⁺
  2. L-threonyl-[rhodopsin] + ATP = O-phospho-L-threonyl-[rhodopsin] + ADP + H⁺

L-seryl-[rhodopsin] + ATP = O-phospho-L-seryl-[rhodopsin] (Bound ligand (Het Group name = SGV) matches with 58.06% similarity) + ADP + H(+)

L-threonyl-[rhodopsin] + ATP = O-phospho-L-threonyl-[rhodopsin] (Bound ligand (Het Group name = SGV) matches with 58.06% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site