PDBsum entry 5wrn

Transferase

PDB id: 5wrn

Contents

Protein chains

(+ 0 more) 282 a.a.

Ligands

DCM ×6

Waters ×328

PDB id:

5wrn

Name:

Transferase

Title:

Human thymidylate synthase complexed with dcmp

Structure:

Thymidylate synthase. Chain: b, a, c, d, e, f. Fragment: unp residues 26-313. Synonym: tsase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tyms, ts, ok/sw-cl.29. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.39Å R-factor: 0.191 R-free: 0.247

Authors:

D.Chen,P.Nordlund

Key ref:

H.Almqvist et al. Human thymidylate synthase complexed with dcmp. To be published, .

Date:

02-Dec-16 Release date: 14-Dec-16

Protein chains

P04818  (TYSY_HUMAN) -  Thymidylate synthase from Homo sapiens

Seq: 313 a.a.

Struc: 282 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.1.1.45 - thymidylate synthase.
• Pathway: Folate Coenzymes
• Reaction: dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP

dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (Bound ligand (Het Group name = DCM) matches with 90.48% similarity) = 7,8-dihydrofolate + dTMP

Molecule diagrams generated from .mol files obtained from the KEGG ftp site