PDBsum entry 4wzm

Transferase

PDB id: 4wzm

Contents

Protein chain

475 a.a.

DNA/RNA

Ligands

GOL ×2

ACT

Metals

_MN

Waters ×35

PDB id:

4wzm

Name:

Transferase

Title:

Mutant k18e of RNA dependent RNA polymerase from foot-and-mouth disease virus complexed with RNA

Structure:

RNA dependent RNA polymerase. Chain: a. Fragment: unp residues 1830-2327. Engineered: yes. Mutation: yes. Other_details: it's the mutant k18e of rdrp from fmdv. RNA template. Chain: b. Engineered: yes.

Source:

Foot-and-mouth disease virus. Fmdv. Organism_taxid: 12120. Strain: isolate -/spain/s8c1santapau/1970 serotypE C. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

2.52Å R-factor: 0.226 R-free: 0.262

Authors:

N.Verdaguer,C.Ferrer-Orta,N.De La Higuera

Key ref:

C.Ferrer-Orta et al. (2015). Multifunctionality of a picornavirus polymerase domain: nuclear localization signal and nucleotide recognition. J Virol, 89, 6848-6859. PubMed id: 25903341 DOI: 10.1128/JVI.03283-14

Date:

20-Nov-14 Release date: 06-May-15

Protein chain

P03311  (POLG_FMDVS) -  Genome polyprotein from Foot-and-mouth disease virus (isolate -/Spain/S8c1SantaPau/1970 serotype C)

Seq: 2327 a.a.

Struc: 475 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-U-G-G-G-C-C-C 8 bases

U-G-G-G-C-C-C 7 bases

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 2: E.C.3.4.22.28 - picornain 3C.
• Reaction: Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 3: E.C.3.4.22.46 - L-peptidase.
• Reaction: Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
• Enzyme class 4: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1128/JVI.03283-14

J Virol 89:6848-6859 (2015)

PubMed id: 25903341

Multifunctionality of a picornavirus polymerase domain: nuclear localization signal and nucleotide recognition.

C.Ferrer-Orta, I.de la Higuera, F.Caridi, M.T.Sánchez-Aparicio, E.Moreno, C.Perales, K.Singh, S.G.Sarafianos, F.Sobrino, E.Domingo, N.Verdaguer.

ABSTRACT

No abstract given.