PDBsum entry 4d0d

Immune system

PDB id: 4d0d

Contents

Protein chains

275 a.a.

96 a.a.

Ligands

VAL-ILE-PHE-PRO-ALA-LYS-SER-LEU ×4

Waters ×9

PDB id:

4d0d

Name:

Immune system

Title:

Complex of a b2 chicken mhc class i molecule and a 8mer chicken peptide

Structure:

Major histocompatibility complex class i glycoprotein haplotype b2. Chain: a, d, g, j. Fragment: extracellular domains, residues 22-293. Engineered: yes. Beta-2-microglobulin. Chain: b, e, h, k. Fragment: residues 22-119. Engineered: yes.

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: plyss rosetta. Other_details: b2 haplotype. Synthetic: yes. Organism_taxid: 9031

Resolution:

3.13Å R-factor: 0.293 R-free: 0.299

Authors:

P.E.Chappell,P.Roversi,M.C.Harrison,L.E.Mears,J.F.Kaufman,S.M.Lea

Key ref:

P.Chappell et al. (2015). Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding. Elife, 4, e05345. PubMed id: 25860507 DOI: 10.7554/eLife.05345

Date:

25-Apr-14 Release date: 06-May-15

Protein chains

O46789  (O46789_CHICK) -  MHC class I alpha chain 2 from Gallus gallus

Seq: 355 a.a.

Struc: 275 a.a.*

Protein chains

P21611  (B2MG_CHICK) -  Beta-2-microglobulin from Gallus gallus

Seq: 119 a.a.

Struc: 96 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DOI no: 10.7554/eLife.05345

Elife 4:e05345 (2015)

PubMed id: 25860507

Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding.

P.Chappell, e.l. .K.Meziane, M.Harrison, �.�.Magiera, C.Hermann, L.Mears, A.G.Wrobel, C.Durant, L.L.Nielsen, S.Buus, N.Ternette, W.Mwangi, C.Butter, V.Nair, T.Ahyee, R.Duggleby, A.Madrigal, P.Roversi, S.M.Lea, J.Kaufman.

ABSTRACT

Highly polymorphic major histocompatibility complex (MHC) molecules are at the heart of adaptive immune responses, playing crucial roles in many kinds of disease and in vaccination. We report that breadth of peptide presentation and level of cell surface expression of class I molecules are inversely correlated in both chickens and humans. This relationship correlates with protective responses against infectious pathogens including Marek's disease virus leading to lethal tumours in chickens and human immunodeficiency virus infection progressing to AIDS in humans. We propose that differences in peptide binding repertoire define two groups of MHC class I molecules strategically evolved as generalists and specialists for different modes of pathogen resistance. We suggest that differences in cell surface expression level ensure the development of optimal peripheral T cell responses. The inverse relationship of peptide repertoire and expression is evidently a fundamental property of MHC molecules, with ramifications extending beyond immunology and medicine to evolutionary biology and conservation.