PDBsum entry 3vht

Fluorescent protein/protein binding

PDB id: 3vht

Contents

Protein chains

229 a.a.

260 a.a.

76 a.a.

Metals

_ZN

Waters ×133

PDB id:

3vht

Name:

Fluorescent protein/protein binding

Title:

Crystal structure of gfp-wrnip1 ubz domain fusion protein in complex with ubiquitin

Structure:

Green fluorescent protein. Chain: a. Fragment: gfp domain. Engineered: yes. Green fluorescent protein,atpase wrnip1. Chain: b. Fragment: gfp,wrnip1 ubz domain (unp residues 9-46). Synonym: werner helicase-interacting protein 1. Engineered: yes.

Source:

Aequorea victoria. Jellyfish. Organism_taxid: 6100. Gene: gfp. Expressed in: escherichia coli. Expression_system_taxid: 469008. Aequorea victoria, homo sapiens. Jellyfish, human. Organism_taxid: 6100, 9606.

Resolution:

2.40Å R-factor: 0.194 R-free: 0.238

Authors:

N.Suzuki,S.Wakatsuki,M.Kawasaki

Key ref:

N.Suzuki et al. (2016). A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1. Febs J, 283, 2004-2017. PubMed id: 27062441 DOI: 10.1111/febs.13734

Date:

06-Sep-11 Release date: 10-Oct-12

Protein chain

P42212  (GFP_AEQVI) -  Green fluorescent protein from Aequorea victoria

Seq: 238 a.a.

Struc: 229 a.a.*

Protein chain

P42212  (GFP_AEQVI) -  Green fluorescent protein from Aequorea victoria

Seq: 238 a.a.

Struc: 260 a.a.*

Protein chain

Q96S55  (WRIP1_HUMAN) -  ATPase WRNIP1 from Homo sapiens

Seq: 665 a.a.

Struc: 260 a.a.*

Protein chain

P62983  (RS27A_MOUSE) -  Ubiquitin-ribosomal protein eS31 fusion protein from Mus musculus

Seq: 156 a.a.

Struc: 76 a.a.

* PDB and UniProt seqs differ at 25 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain B: E.C.3.6.1.- - ?????
• Enzyme class 3: Chain B: E.C.3.6.1.3 - Deleted entry.
• Reaction: ATP + H₂O = ADP + phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1111/febs.13734

Febs J 283:2004-2017 (2016)

PubMed id: 27062441

A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1.

N.Suzuki, A.Rohaim, R.Kato, I.Dikic, S.Wakatsuki, M.Kawasaki.

ABSTRACT

No abstract given.