PDBsum entry 2wnn

Lyase

PDB id: 2wnn

Contents

Protein chains

296 a.a. *

Ligands

1PE ×3

Metals

_NA

Waters ×758

* Residue conservation analysis

PDB id:

2wnn

Name:

Lyase

Title:

Structure of wild type e. Coli n-acetylneuraminic acid lyase in complex with pyruvate in space group p21

Structure:

N-acetylneuraminate lyase. Chain: a, b, c, d. Fragment: residues 2-296. Synonym: n-acetylneuraminic acid lyase, n-acetylneuraminate pyruvate- lyase, sialic acid lyase, sialate lyase, sialic acid aldolase, nalase. Engineered: yes. Other_details: schiff base between lys165 and pyruvate in all chains

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.65Å R-factor: 0.212 R-free: 0.249

Authors:

I.Campeotto,A.H.Bolt,T.A.Harman,C.H.Trinh,C.A.Dennis,S.E.V.Phillips, A.R.Pearson,A.Nelson,A.Berry

Key ref:

I.Campeotto et al. (2010). Structural insights into substrate specificity in variants of N-acetylneuraminic Acid lyase produced by directed evolution. J Mol Biol, 404, 56-69. PubMed id: 20826162

Date:

13-Jul-09 Release date: 25-Aug-10

Protein chains

P0A6L4  (NANA_ECOLI) -  N-acetylneuraminate lyase from Escherichia coli (strain K12)

Seq: 297 a.a.

Struc: 296 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.1.3.3 - N-acetylneuraminate lyase.
• Reaction: aceneuramate = aldehydo-N-acetyl-D-mannosamine + pyruvate

aceneuramate = aldehydo-N-acetyl-D-mannosamine + pyruvate (Bound ligand (Het Group name = 1PE) matches with 44.44% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

J Mol Biol 404:56-69 (2010)

PubMed id: 20826162

Structural insights into substrate specificity in variants of N-acetylneuraminic Acid lyase produced by directed evolution.

I.Campeotto, A.H.Bolt, T.A.Harman, C.Dennis, C.H.Trinh, S.E.Phillips, A.Nelson, A.R.Pearson, A.Berry.

ABSTRACT

No abstract given.