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UniProt functional annotation for P0A6L4 | ||
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| UniProt code: P0A6L4. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate. |
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| Catalytic activity: | |
Reaction=N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate; Xref=Rhea:RHEA:23296, ChEBI:CHEBI:15361, ChEBI:CHEBI:17122, ChEBI:CHEBI:35418; EC=4.1.3.3; |
| Activity regulation: | |
Inhibited by reduction with NaBH(4), and by Cu(2+) ions, p-chloromercuribenzoate and N-bromosuccinimide. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 6.5-7.0.; Temperature dependence: Optimum temperature is 80 degrees Celsius.; |
| Pathway: | |
Amino-sugar metabolism; N-acetylneuraminate degradation; D- fructose 6-phosphate from N-acetylneuraminate: step 1/5. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:8081752}. |
| Subcellular location: | |
Cytoplasm. |
| Induction: | |
Negatively regulated by the transcriptional repressor NanR. Induced by N-acetylneuraminate, via inactivation of NanR. {ECO:0000269|PubMed:23935044}. |
| Similarity: | |
Belongs to the DapA family. NanA subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.