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PDBsum entry 1tcs
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Protein synthesis inhibitor
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PDB id
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1tcs
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Contents |
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* Residue conservation analysis
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Enzyme class:
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E.C.3.2.2.22
- rRNA N-glycosylase.
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Reaction:
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Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
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Nat Struct Biol
1:695-700
(1994)
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PubMed id:
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Crystal structure of trichosanthin-NADPH complex at 1.7 A resolution reveals active-site architecture.
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J.P.Xiong,
Z.X.Xia,
Y.Wang.
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ABSTRACT
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We describe here the crystal structure of the trichosanthin-NADPH complex
determined at a resolution of 1.7 A. The adenine base stacks between Tyr 70 and
Tyr 111. Arg 163, Glu 160 and Tyr 70 form hydrogen bonds to N(3), O(3') and,
through a water molecule, to N(9) of adenosine, respectively. This is the first
high resolution structure of a complex between a ribosome-inactivating protein
and a substrate analogue, in which the electron density of the N-glycosidic bond
is well defined and the preassociated water, thought to be responsible for
hydrolyzing the N-C bond, is also explicitly elucidated.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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A.Kumar,
N.K.Singhal,
B.Ramanujam,
A.Mitra,
N.R.Rameshwaram,
S.K.Nadimpalli,
and
C.P.Rao
(2009).
C(1)-/C(2)-aromatic-imino-glyco-conjugates: experimental and computational studies of binding, inhibition and docking aspects towards glycosidases isolated from soybean and jack bean.
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Glycoconj J,
26,
495-510.
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D.S.Chan,
L.O.Chu,
K.M.Lee,
P.H.Too,
K.W.Ma,
K.H.Sze,
G.Zhu,
P.C.Shaw,
and
K.B.Wong
(2007).
Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analyses.
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Nucleic Acids Res,
35,
1660-1672.
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K.Zhang,
J.Xu,
X.Huang,
L.Wu,
C.Wen,
Y.Hu,
Y.Su,
Y.Chen,
and
Z.Zhang
(2007).
Trichosanthin down-regulated p210Bcr-Abl and enhanced imatinib-induced growth arrest in chronic myelogenous leukemia cell line K562.
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Cancer Chemother Pharmacol,
60,
581-587.
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Q.An,
S.Wei,
S.Mu,
X.Zhang,
Y.Lei,
W.Zhang,
N.Jia,
X.Cheng,
A.Fan,
Z.Li,
and
Z.Xu
(2006).
Mapping the antigenic determinants and reducing the immunogenicity of trichosanthin by site-directed mutagenesis.
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J Biomed Sci,
13,
637-643.
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S.Quevillon-Cheruel,
N.Leulliot,
P.Meyer,
M.Graille,
M.Bremang,
K.Blondeau,
I.Sorel,
A.Poupon,
J.Janin,
and
H.van Tilbeurgh
(2004).
Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiae.
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J Biol Chem,
279,
619-625.
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PDB codes:
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M.Hellings,
M.De Maeyer,
S.Verheyden,
Q.Hao,
E.J.Van Damme,
W.J.Peumans,
and
Y.Engelborghs
(2003).
The dead-end elimination method, tryptophan rotamers, and fluorescence lifetimes.
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Biophys J,
85,
1894-1902.
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R.Kho,
B.L.Baker,
J.V.Newman,
R.M.Jack,
D.S.Sem,
H.O.Villar,
and
M.R.Hansen
(2003).
A path from primary protein sequence to ligand recognition.
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Proteins,
50,
589-599.
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Y.J.Gu,
and
Z.X.Xia
(2000).
Crystal structures of the complexes of trichosanthin with four substrate analogs and catalytic mechanism of RNA N-glycosidase.
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Proteins,
39,
37-46.
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PDB code:
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M.H.Eppink,
H.A.Schreuder,
and
W.J.van Berkel
(1998).
Interdomain binding of NADPH in p-hydroxybenzoate hydroxylase as suggested by kinetic, crystallographic and modeling studies of histidine 162 and arginine 269 variants.
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J Biol Chem,
273,
21031-21039.
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PDB codes:
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S.Wu,
X.Lu,
Y.Zhu,
J.Yang,
and
Y.Dong
(1998).
N-glycosidase mechanism of Trichosanthin.
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Sci China C Life Sci,
41,
174-180.
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P.C.Shaw,
S.Mulot,
S.K.Ma,
Q.F.Xu,
H.B.Yao,
S.Wu,
X.H.Lu,
and
Y.C.Dong
(1997).
Structure/function relationship study of Tyr14 and Arg22 in trichosanthin, a ribosome-inactivating protein.
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Eur J Biochem,
245,
423-427.
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J.P.Xiong,
Z.X.Xia,
and
Y.Wang
(1995).
Identification of a stable complex of trichosanthin with nicotinamide adenine dinucleotide phosphate.
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J Protein Chem,
14,
139-144.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
