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PDBsum entry 1fjf
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234 a.a.
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206 a.a.
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208 a.a.
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150 a.a.
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101 a.a.
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155 a.a.
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138 a.a.
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127 a.a.
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98 a.a.
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119 a.a.
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124 a.a.
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125 a.a.
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60 a.a.
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88 a.a.
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83 a.a.
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104 a.a.
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73 a.a.
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80 a.a.
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99 a.a.
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24 a.a.
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References listed in PDB file
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Key reference
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Title
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Structure of the 30s ribosomal subunit.
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Authors
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B.T.Wimberly,
D.E.Brodersen,
W.M.Clemons,
R.J.Morgan-Warren,
A.P.Carter,
C.Vonrhein,
T.Hartsch,
V.Ramakrishnan.
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Ref.
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Nature, 2000,
407,
327-339.
[DOI no: ]
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PubMed id
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Abstract
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Genetic information encoded in messenger RNA is translated into protein by the
ribosome, which is a large nucleoprotein complex comprising two subunits,
denoted 30S and 50S in bacteria. Here we report the crystal structure of the 30S
subunit from Thermus thermophilus, refined to 3 A resolution. The final atomic
model rationalizes over four decades of biochemical data on the ribosome, and
provides a wealth of information about RNA and protein structure, protein-RNA
interactions and ribosome assembly. It is also a structural basis for analysis
of the functions of the 30S subunit, such as decoding, and for understanding the
action of antibiotics. The structure will facilitate the interpretation in
molecular terms of lower resolution structural data on several functional states
of the ribosome from electron microscopy and crystallography.
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Figure 4.
Figure 4: Structure of the 5' domain of 16S RNA. a, Stereo
view of the entire 5' domain, with an inset on the right showing
its location in the 30S subunit. The upper (b), middle (c) and
lower (d) subdomains are shown separately next to corresponding
parts of the secondary structure diagrams. The colours in the
secondary structure diagrams match those in the structure in
this and Figs 5 and 6.
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Figure 6.
Figure 6: Structure of the 3' major and 3' minor domains of 16S
RNA. a, Stereo view of the 3' major domain with inset showing
its location in the 30S. b-d, The upper, middle and lower parts
of the 3' major domain, with corresponding secondary structure
diagrams. e, Stereo view of the 3' minor domain, with secondary
structure diagram and inset showing its location in the 30S.
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The above figures are
reprinted
by permission from Macmillan Publishers Ltd:
Nature
(2000,
407,
327-339)
copyright 2000.
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Secondary reference #1
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Title
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Functional insights from the structure of the 30s ribosomal subunit and its interactions with antibiotics.
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Authors
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A.P.Carter,
W.M.Clemons,
D.E.Brodersen,
R.J.Morgan-Warren,
B.T.Wimberly,
V.Ramakrishnan.
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Ref.
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Nature, 2000,
407,
340-348.
[DOI no: ]
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PubMed id
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Figure 4.
Figure 4: Interaction of spectinomycin with the 30S ribosomal
subunit. a, Difference Fourier maps showing the binding site
of spectinomycin in helix 34. b, Chemical structure of
spectinomycin, showing interactions of the various groups with
specific residues of 30S. c, The spectinomycin-binding site,
showing its location at a pivotal point in the head of the 30S
subunit. d, Inset showing spectinomycin in a space-filling
model, and the location of its binding site on the 30S.
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Figure 5.
Figure 5: Interaction of streptomycin with the 30S ribosomal
subunit. a, Difference Fourier maps showing the binding site
of streptomycin. Mutations in ribosomal protein S12 that confer
resistance are shown in red. b, Chemical structure of
streptomycin, showing interactions of the various groups with
specific residues of the ribosome. c, The streptomycin-binding
site, showing its interaction with H27, the 530 loop (H18), H44
and ribosomal protein S12. d, A view of the 30S showing
streptomycin in a space-filling model, and the surrounding RNA
and protein elements.
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The above figures are
reproduced from the cited reference
with permission from Macmillan Publishers Ltd
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Secondary reference #2
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Title
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Structure of a bacterial 30s ribosomal subunit at 5.5 a resolution.
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Authors
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W.M.Clemons,
J.L.May,
B.T.Wimberly,
J.P.Mccutcheon,
M.S.Capel,
V.Ramakrishnan.
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Ref.
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Nature, 1999,
400,
833-840.
[DOI no: ]
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PubMed id
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Figure 4.
Figure 4 Stereo view of the three-way junction formed by
helices 20, 21 and 22 of the central domain of 16S RNA. Inset
(right) shows the structure in the context of the 30S subunit.
Figure made with RIBBONS50.
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Figure 5.
Figure 5 Stereo view of the interactions made by helix 27 of
the central domain with helices 24 and 44 of 16S RNA. Inset
(right) shows the elements in the whole 30S subunit. Figure made
with RIBBONS50.
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The above figures are
reproduced from the cited reference
with permission from Macmillan Publishers Ltd
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