The enzyme catalyzes the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in oligo-mannose oligosaccarides. It plays a critical role in the maturation of Asn-linked glycoproteins and triggers the signal for degradation of misfolded glycoproteins. It binds an essential calcium ion (PMID10995765).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| CA 901 x | CA | calcium | calcium | mononuclear | Coordinates substrate |
*It refers to the MACiE reference pdb: 1dl2
| Metal/s Properties in Resting State | ||||||
| CA 901 x | Resting state enzyme (1fmi) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | 8 | |||||
| Notes | - | |||||
| References |
| -Karaveg K, Siriwardena A, Tempel W, Liu ZJ, Glushka J, Wang BC, Moremen KW Mechanism of class 1 (glycosylhydrolase family 47) {alpha}-mannosidases involved in N-glycan processing and endoplasmic reticulum quality control. J Biol Chem. 2005 Apr 22;280(16):16197-207. Epub 2005 Feb 15.(MEDLINE:15713668) |
| - Some information have been also deduced from the MACiE mechanism model |