PDBsum entry 9b8r

DNA binding protein/DNA

PDB id: 9b8r

Contents

Protein chain

1119 a.a.

DNA/RNA

Ligands

SF4

PDB id:

9b8r

Name:

DNA binding protein/DNA

Title:

Cryo-em structure of s. Cerevisiae pole-core-DNA

Structure:

Primer DNA. Chain: p. Engineered: yes. Template DNA. Chain: t. Engineered: yes. DNA polymerase epsilon catalytic subunit. Chain: e. Engineered: yes

Source:

Synthetic: yes. DNA molecule. Organism_taxid: 2853804. Saccharomyces cerevisiae. Brewer's yeast. Organism_taxid: 4932. Gene: pol2, gi527_g0004851. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932

Authors:

Z.Yuan,R.Georgescu,M.O'Donnell,H.Li

Key ref:

Z.Yuan et al. (2024). Mechanism of pcna loading by ctf18-Rfc for leading-St DNA synthesis. Science, 385, . PubMed id: 39088616

Date:

31-Mar-24 Release date: 09-Oct-24

Protein chain

P21951  (DPOE_YEAST) -  DNA polymerase epsilon catalytic subunit A from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 2222 a.a.

Struc: 1119 a.a.

DNA/RNA chains

C-T-G-T-T-G-C-T-G-C 10 bases

T-A-G-C-A-G-C-A-A-C-A-G 12 bases

Enzyme reactions

• Enzyme class 2: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.1.11.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site