PDBsum entry 8v9c

Viral protein/inhibitor

PDB id: 8v9c

Contents

Protein chains

209 a.a.

Ligands

2SQ ×2

PDB id:

8v9c

Name:

Viral protein/inhibitor

Title:

HIV-1 integrase f185h complexed with allosteric inhibitor gsk1264

Structure:

Integrase. Chain: a, b. Synonym: in. Engineered: yes. Mutation: yes

Source:

Human immunodeficiency virus 1. Organism_taxid: 11676. Strain: nl4-3. Gene: gag-pol. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

4.40Å R-factor: 0.318 R-free: 0.341

Authors:

S.Montermoso,K.Gupta,G.Eilers,F.D.Bushman,G.D.Van Duyne

Key ref:

S.Montermoso et al. Structural consequences of resistance mutations on th formation of allini-Induced branched polymers of HIV- Integrase. To be published, . PubMed id: 40409709

Date:

07-Dec-23 Release date: 19-Mar-25

Supersedes:

5hot

Protein chains

P12497  (POL_HV1N5) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate NY5)

Seq: 1435 a.a.

Struc: 209 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.- - ?????
• Enzyme class 2: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: E.C.3.1.-.-
• Enzyme class 5: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 7: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site