PDBsum entry 8tk2

Hydrolase

PDB id: 8tk2

Contents

Protein chains

181 a.a.

Ligands

I1Y ×2

DMS

PEG

Waters ×240

PDB id:

8tk2

Name:

Hydrolase

Title:

Human vh1-related dual-specificity phosphatase (vhr) complexed with 2- ((2,4-difluorobenzyl)amino)-2-oxoacetic acid

Structure:

Dual specificity protein phosphatase 3. Chain: a, b. Synonym: dual specificity protein phosphatase vhr,vaccinia h1-related phosphatase,vhr. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: dusp3, vhr. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.70Å R-factor: 0.179 R-free: 0.223

Authors:

A.E.Aleshin,J.Wu,L.J.Lambert,N.D.P.Cosford,L.Tautz

Key ref:

A.E.Aleshin et al. Human vh1-Related dual-Specificity phosphatase (vhr) 2-((2,4-Difluorobenzyl)amino)-2-Oxoacetic acid. To be published, . PubMed id: 39959108

Date:

25-Jul-23 Release date: 14-Aug-24

Protein chains

P51452  (DUS3_HUMAN) -  Dual specificity protein phosphatase 3 from Homo sapiens

Seq: 185 a.a.

Struc: 181 a.a.

Enzyme reactions

• Enzyme class 2: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate
• Enzyme class 3: E.C.3.1.3.48 - protein-tyrosine-phosphatase.
• Reaction: O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site