PDBsum entry 8hg1

Replication/DNA

PDB id: 8hg1

Contents

Protein chains

1005 a.a.

218 a.a.

419 a.a.

DNA/RNA

Ligands

TTP

Metals

_MG

PDB id:

8hg1

Name:

Replication/DNA

Title:

The structure of mpxv polymerase holoenzyme in replicating state

Structure:

DNA polymerase. Chain: a. Engineered: yes. E4r. Chain: b. Synonym: mpxv-cop-095,mpxv-sl-095,mpxv-wrair095,mpxvgp101,uracil-DNA glycosylase,uracil-DNA glycosylase DNA polymerase,uracil-DNA glycosylase interacts with a20r,DNA polymerase processivity factor. Engineered: yes.

Source:

Monkeypox virus. Monkeypox. Organism_taxid: 10244. Gene: pol, mpxv-m2940_fct-055, mpxv-m2957_lagos-055, mpxv- m3021_delta-055, mpxv-m5320_m15_bayelsa-048, mpxv-singapore-055, mpxv-uk_p1-055, mpxv-uk_p2-055, mpxv-uk_p3-055, mpxv298464_038, pdlmklco_00060. Expressed in: spodoptera. Expression_system_taxid: 7106.

Authors:

Q.Peng,Y.F.Xie,L.Kuai,H.Wang,J.X.Qi,F.Gao,Y.Shi

Key ref:

Q.Peng et al. (2023). Structure of monkeypox virus DNA polymerase holoenzym. Science, 379, 100. PubMed id: 36520947

Date:

13-Nov-22 Release date: 21-Dec-22

Protein chain

A0A7H0DN44  (DPOL_MONPV) -  DNA polymerase from Monkeypox virus

Seq: 1006 a.a.

Struc: 1005 a.a.*

Protein chain

M1LL92  (UNG_MONPV) -  Uracil-DNA glycosylase from Monkeypox virus

Seq: 218 a.a.

Struc: 218 a.a.

Protein chain

A0A7H0DNC0  (PG148_MONPV) -  DNA polymerase processivity factor component OPG148 from Monkeypox virus

Seq: 426 a.a.

Struc: 419 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

T-G-A-T-T-A-C-G-A-A-T-T-G-C 14 bases

C-G-A-A-T-T-A-A-G-C-A-A-T-T-C-G-T-A-A-T-C-A 22 bases

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 2: Chain B: E.C.3.2.2.27 - uracil-DNA glycosylase.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site