PDBsum entry 8fcc

Viral protein

PDB id: 8fcc

Contents

Protein chains

543 a.a.

400 a.a.

Ligands

YO9

TLA

Waters ×110

PDB id:

8fcc

Name:

Viral protein

Title:

HIV-1 reverse transcriptase in complex with 5-membered bicyclic core nnrti

Structure:

P66 rt. Chain: a. Synonym: reverse transcriptase/ribonuclease h,exoribonuclease h,p66 rt. Ec: 2.7.7.49,2.7.7.7,3.1.26.13,3.1.13.2. Engineered: yes. P51 rt. Chain: b. Engineered: yes

Source:

HIV whole-genome vector aa1305#18. Organism_taxid: 672471. Gene: gag-pol. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.57Å R-factor: 0.203 R-free: 0.257

Authors:

E.B.Lansdon

Key ref:

L.Prener et al. Design and synthesis of novel HIV-1 nnrtis with bicyc cores and with improved physicochemical properties.. J med chem, . PubMed id: 36652602

Date:

01-Dec-22 Release date: 01-Feb-23

Protein chain

P04585  (POL_HV1H2) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)

Seq: 1435 a.a.

Struc: 543 a.a.

Protein chain

P04585  (POL_HV1H2) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)

Seq: 1435 a.a.

Struc: 400 a.a.

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.2.7.7.- - ?????
• Enzyme class 2: Chains A, B: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: Chains A, B: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: Chains A, B: E.C.3.1.-.-
• Enzyme class 5: Chains A, B: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: Chains A, B: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 7: Chains A, B: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site