PDBsum entry 8efc

DNA binding protein

PDB id: 8efc

Contents

Protein chain

595 a.a.

DNA/RNA

Ligands

DGT

Metals

_MG

PDB id:

8efc

Name:

DNA binding protein

Title:

Structure of lates calcarifer DNA polymerase theta polymerase domain with long duplex DNA, complex ia

Structure:

DNA polymerase theta. Chain: a. Engineered: yes. DNA (5'- d( Ap Gp Cp Tp Cp Tp Ap Cp Gp Gp Ap Tp Gp Cp Cp Tp Cp Ap Cp Ap G)- 3'). Chain: e. Engineered: yes. DNA (5'-

Source:

Lates calcarifer. Organism_taxid: 8187. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768. Synthetic: yes. Organism_taxid: 8187

Authors:

C.Li,H.Zhu,J.Sun,Y.Gao

Key ref:

C.Li et al. Structural basis of DNA polymerase theta mediated DNA joining.. Nucleic acids res, . PubMed id: 36583344

Date:

08-Sep-22 Release date: 14-Dec-22

Protein chain

A0A4W6BKE5  (A0A4W6BKE5_LATCA) -  DNA-directed DNA polymerase from Lates calcarifer

Seq: 2593 a.a.

Struc: 595 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

DNA/RNA chains

A-G-C-T-C-T-A-C-G-G-A-T-G-C-C-T-C-A-C-A-G 21 bases

A-C-T-G-T-G-A-G-G-C-A-T-C-C-G-T-A-G-2DA 19 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site