PDBsum entry 7xpy

Hydrolase

PDB id: 7xpy

Contents

Protein chain

528 a.a.

Ligands

EIB

Waters ×29

PDB id:

7xpy

Name:

Hydrolase

Title:

Crystal structure of usp7 in complex with its inhibitor

Structure:

Ubiquitin carboxyl-terminal hydrolase 7. Chain: a. Fragment: unp residues 560-1102. Synonym: deubiquitinating enzyme 7,herpesvirus-associated ubiquitin- specific protease,ubiquitin thioesterase 7,ubiquitin-specific- processing protease 7. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: usp7, hausp. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.35Å R-factor: 0.226 R-free: 0.266

Authors:

N.Feng,K.W.Zeng

Key ref:

X.W.Zhang et al. (2022). Neuroinflammation inhibition by small-Molecule target usp7 noncatalytic domain for neurodegenerative diseas therapy.. Sci adv, 8, o0789. PubMed id: 35947662

Date:

06-May-22 Release date: 24-Aug-22

Protein chain

Q93009  (UBP7_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 7 from Homo sapiens

Seq: 1102 a.a.

Struc: 528 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).