PDBsum entry 7o2m

Viral protein

PDB id: 7o2m

Contents

Protein chains

38 a.a.

154 a.a.

Ligands

UZZ

Waters ×110

PDB id:

7o2m

Name:

Viral protein

Title:

Crystal structure of unlinked ns2b-ns3 protease from zika virus in complex with inhibitor mi-2289

Structure:

Genome polyprotein. Chain: a. Engineered: yes. Genome polyprotein. Chain: b. Engineered: yes

Source:

Zika virus. Zikv. Organism_taxid: 64320. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.187 R-free: 0.251

Authors:

S.Huber,A.Heine,T.Steinmetzer

Key ref:

S.Huber et al. Optimization of macrocyclic inhibitors of the ns2b-Ns protease of zika virus. To be published, . PubMed id: 35475620

Date:

30-Mar-21 Release date: 06-Apr-22

Protein chain

Q32ZE1  (POLG_ZIKV) -  Genome polyprotein from Zika virus

Seq: 3419 a.a.

Struc: 38 a.a.

Protein chain

Q32ZE1  (POLG_ZIKV) -  Genome polyprotein from Zika virus

Seq: 3419 a.a.

Struc: 154 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.2.1.1.56 - mRNA (guanine-N(7))-methyltransferase.
• Reaction: a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine
• Enzyme class 2: Chains A, B: E.C.2.1.1.57 - methyltransferase cap1.
• Reaction: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)- (2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H⁺
• Enzyme class 3: Chains A, B: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 4: Chains A, B: E.C.3.4.21.91 - flavivirin.
• Reaction: Selective hydrolysis of Xaa-Xaa-|-Xbb bonds in which each of the Xaa can be either Arg or Lys and Xbb can be either Ser or Ala.
• Enzyme class 5: Chains A, B: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺
• Enzyme class 6: Chains A, B: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site