PDBsum entry 7nhq

Photosynthesis

PDB id: 7nhq

Contents

Protein chains

335 a.a.

496 a.a.

432 a.a.

341 a.a.

77 a.a.

38 a.a.

65 a.a.

26 a.a.

37 a.a.

37 a.a.

34 a.a.

28 a.a.

35 a.a.

28 a.a.

60 a.a.

112 a.a.

56 a.a.

Ligands

PHO ×2

CLA ×35

BCR ×10

LHG

LMG ×7

PL9

HEM

Metals

_FE

_MN

_CL

PDB id:

7nhq

Name:

Photosynthesis

Title:

Structure of psii-i prime (psii with psb28, and psb34)

Structure:

Photosystem ii protein d1 1. Chain: a. Synonym: psii d1 protein 1,photosystem ii q(b) protein 1. Photosystem ii cp47 reaction center protein. Chain: b. Synonym: psii 47 kda protein,protein cp-47. Photosystem ii cp43 reaction center protein. Chain: c. Synonym: psii 43 kda protein,protein cp-43.

Source:

Thermosynechococcus elongatus bp-1. Organism_taxid: 197221. Organism_taxid: 197221

Authors:

J.Zabret,S.Bohn,S.K.Schuller,O.Arnolds,A.Chan,E.Tajkhorshid,R.Stoll, B.D.Engel,T.Rudack,J.M.Schuller,M.M.Nowaczyk

Key ref:

J.Zabret et al. (2021). Structural insights into photosystem II assembly. Nat Plants, 7, 524-538. PubMed id: 33846594 DOI: 10.1107/S2059798319011471

Date:

11-Feb-21 Release date: 05-May-21

Protein chain

P0A444  (PSBA1_THEEB) -  Photosystem II protein D1 1 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 360 a.a.

Struc: 335 a.a.

Protein chain

Q8DIQ1  (PSBB_THEEB) -  Photosystem II CP47 reaction center protein from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 510 a.a.

Struc: 496 a.a.

Protein chain

Q8DIF8  (PSBC_THEEB) -  Photosystem II CP43 reaction center protein from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 461 a.a.

Struc: 432 a.a.

Protein chain

Q8CM25  (PSBD_THEEB) -  Photosystem II D2 protein from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 352 a.a.

Struc: 341 a.a.

Protein chain

Q8DIP0  (PSBE_THEEB) -  Cytochrome b559 subunit alpha from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 84 a.a.

Struc: 77 a.a.

Protein chain

Q8DIN9  (PSBF_THEEB) -  Cytochrome b559 subunit beta from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 45 a.a.

Struc: 38 a.a.

Protein chain

Q8DJ43  (PSBH_THEEB) -  Photosystem II reaction center protein H from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 66 a.a.

Struc: 65 a.a.

Protein chain

Q8DJZ6  (PSBI_THEEB) -  Photosystem II reaction center protein I from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 38 a.a.

Struc: 26 a.a.

Protein chain

Q9F1K9  (PSBK_THEEB) -  Photosystem II reaction center protein K from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 46 a.a.

Struc: 37 a.a.

Protein chain

Q8DIN8  (PSBL_THEEB) -  Photosystem II reaction center protein L from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 37 a.a.

Struc: 37 a.a.

Protein chain

Q8DHA7  (PSBM_THEEB) -  Photosystem II reaction center protein M from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 36 a.a.

Struc: 34 a.a.

Protein chain

Q8DIQ0  (PSBT_THEEB) -  Photosystem II reaction center protein T from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 32 a.a.

Struc: 28 a.a.

Protein chain

Q9F1R6  (PSBX_THEEB) -  Photosystem II reaction center protein X from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 41 a.a.

Struc: 35 a.a.

Protein chain

Q8DJI1  (YCF12_THEEB) -  Photosystem II reaction center protein Psb30 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 46 a.a.

Struc: 28 a.a.

Protein chain

Q8DHJ2  (PSBZ_THEEB) -  Photosystem II reaction center protein Z from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 62 a.a.

Struc: 60 a.a.

Protein chain

Q8DLJ8  (PSB28_THEEB) -  Photosystem II assembly factor Psb28 protein from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 116 a.a.

Struc: 112 a.a.

Protein chain

Q8DMP8  (PSB34_THEVB) -  Photosystem II assembly protein Psb34 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 56 a.a.

Struc: 56 a.a.

Enzyme reactions

• Enzyme class 2: Chains B, C, E, F, H, I, K, L, M, T, X, y, Z, 2, 3: E.C.?
• Enzyme class 3: Chains A, D: E.C.1.10.3.9 - photosystem Ii.
• Reaction: 2 a plastoquinone + 4 hnu + 2 H2O = 2 a plastoquinol + O2

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1107/S2059798319011471

Nat Plants 7:524-538 (2021)

PubMed id: 33846594

Structural insights into photosystem II assembly.

J.Zabret, S.Bohn, S.K.Schuller, O.Arnolds, M.Möller, J.Meier-Credo, P.Liauw, A.Chan, E.Tajkhorshid, J.D.Langer, R.Stoll, A.Krieger-Liszkay, B.D.Engel, T.Rudack, J.M.Schuller, M.M.Nowaczyk.

ABSTRACT

Biogenesis of photosystem II (PSII), nature's water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. Using cryo-electron microscopy, we solved the structure of such a PSII assembly intermediate from Thermosynechococcus elongatus at 2.94 Å resolution. It contains three assembly factors (Psb27, Psb28 and Psb34) and provides detailed insights into their molecular function. Binding of Psb28 induces large conformational changes at the PSII acceptor side, which distort the binding pocket of the mobile quinone (Q_B) and replace the bicarbonate ligand of non-haem iron with glutamate, a structural motif found in reaction centres of non-oxygenic photosynthetic bacteria. These results reveal mechanisms that protect PSII from damage during biogenesis until water splitting is activated. Our structure further demonstrates how the PSII active site is prepared for the incorporation of the Mn₄CaO₅ cluster, which performs the unique water-splitting reaction.