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PDBsum entry 7nhq
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Photosynthesis
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PDB id
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7nhq
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Contents |
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335 a.a.
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496 a.a.
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432 a.a.
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341 a.a.
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77 a.a.
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38 a.a.
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65 a.a.
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26 a.a.
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37 a.a.
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37 a.a.
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34 a.a.
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28 a.a.
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35 a.a.
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28 a.a.
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60 a.a.
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112 a.a.
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56 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural insights into photosystem ii assembly.
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Authors
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J.Zabret,
S.Bohn,
S.K.Schuller,
O.Arnolds,
M.Möller,
J.Meier-Credo,
P.Liauw,
A.Chan,
E.Tajkhorshid,
J.D.Langer,
R.Stoll,
A.Krieger-Liszkay,
B.D.Engel,
T.Rudack,
J.M.Schuller,
M.M.Nowaczyk.
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Ref.
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Nat Plants, 2021,
7,
524-538.
[DOI no: ]
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PubMed id
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Abstract
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Biogenesis of photosystem II (PSII), nature's water-splitting catalyst, is
assisted by auxiliary proteins that form transient complexes with PSII
components to facilitate stepwise assembly events. Using cryo-electron
microscopy, we solved the structure of such a PSII assembly intermediate from
Thermosynechococcus elongatus at 2.94 Å resolution. It contains three
assembly factors (Psb27, Psb28 and Psb34) and provides detailed insights into
their molecular function. Binding of Psb28 induces large conformational changes
at the PSII acceptor side, which distort the binding pocket of the mobile
quinone (QB) and replace the bicarbonate ligand of non-haem iron with
glutamate, a structural motif found in reaction centres of non-oxygenic
photosynthetic bacteria. These results reveal mechanisms that protect PSII from
damage during biogenesis until water splitting is activated. Our structure
further demonstrates how the PSII active site is prepared for the incorporation
of the Mn4CaO5 cluster, which performs the unique
water-splitting reaction.
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