PDBsum entry 7auh

Hydrolase

PDB id: 7auh

Contents

Protein chain

503 a.a.

Ligands

GOL

4D6

Waters ×109

PDB id:

7auh

Name:

Hydrolase

Title:

Structure of p. Aeruginosa pbp3 in complex with vaborbactam

Structure:

Peptidoglycan d,d-transpeptidase ftsi. Chain: a. Synonym: penicillin-binding protein 3,pbp-3. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1. Gene: ftsi, pbpb, pa4418. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.01Å R-factor: 0.208 R-free: 0.281

Authors:

H.Newman,B.Bellini,C.G.Dowson

Key ref:

H.Newman et al. (2021). High-Throughput crystallography reveals boron-Contain inhibitors of a penicillin-Binding protein with di- A tricovalent binding modes.. J med chem, 64, 11379. PubMed id: 34337941

Date:

03-Nov-20 Release date: 11-Aug-21

Protein chain

G3XD46  (FTSI_PSEAE) -  Peptidoglycan D,D-transpeptidase FtsI from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 579 a.a.

Struc: 503 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.16.4 - serine-type D-Ala-D-Ala carboxypeptidase.
• Reaction: D-alanyl-D-alanine + H₂O = 2 D-alanine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site