PDBsum entry 6hqb

Photosynthesis

PDB id: 6hqb

Contents

Protein chains

751 a.a.

731 a.a.

80 a.a.

141 a.a.

69 a.a.

143 a.a.

40 a.a.

40 a.a.

70 a.a.

137 a.a.

31 a.a.

Ligands

CLA ×93

PQN ×2

SF4 ×3

BCR ×14

LHG ×4

45D

ECH ×2

LMG ×2

SQD ×2

C7Z ×2

LMT

EQ3

Metals

_CL

_CA ×2

PDB id:

6hqb

Name:

Photosynthesis

Title:

Monomeric cyanobacterial photosystem i

Structure:

Photosystem i p700 chlorophyll a apoprotein a1. Chain: a. Synonym: psaa. Photosystem i p700 chlorophyll a apoprotein a2. Chain: b. Synonym: psab. Photosystem i iron-sulfur center. Chain: c. Synonym: 9 kda polypeptide,psi-c,photosystem i subunit vii,psac.

Source:

Synechocystis sp. (Strain pcc 6803 / kazusa). Organism_taxid: 1111708. Strain: pcc 6803 / kazusa. Strain: pcc 6803 / kazusa

Resolution:

4.00Å R-factor: 0.256 R-free: 0.295

Authors:

S.Y.Netzer-El,N.Nelson,I.Caspy

Key ref:

S.Y.Netzer-El et al. (2018). Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803. Front Plant Sci, 9, 1865. PubMed id: 30662446 DOI: 10.3389/fpls.2018.01865

Date:

24-Sep-18 Release date: 16-Jan-19

Protein chain

P29254  (PSAA_SYNY3) -  Photosystem I P700 chlorophyll a apoprotein A1 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 751 a.a.

Struc: 751 a.a.

Protein chain

P29255  (PSAB_SYNY3) -  Photosystem I P700 chlorophyll a apoprotein A2 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 731 a.a.

Struc: 731 a.a.

Protein chain

P32422  (PSAC_SYNY3) -  Photosystem I iron-sulfur center from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 81 a.a.

Struc: 80 a.a.

Protein chain

P19569  (PSAD_SYNY3) -  Photosystem I reaction center subunit II from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 141 a.a.

Struc: 141 a.a.

Protein chain

P12975  (PSAE_SYNY3) -  Photosystem I reaction center subunit IV from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 74 a.a.

Struc: 69 a.a.

Protein chain

P29256  (PSAF_SYNY3) -  Photosystem I reaction center subunit III from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 165 a.a.

Struc: 143 a.a.

Protein chain

Q55330  (PSAI_SYNY3) -  Photosystem I reaction center subunit VIII from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 40 a.a.

Struc: 40 a.a.

Protein chain

Q55329  (PSAJ_SYNY3) -  Photosystem I reaction center subunit IX from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 40 a.a.

Struc: 40 a.a.

Protein chain

P74564  (PSAK2_SYNY3) -  Photosystem I reaction center subunit PsaK 2 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 90 a.a.

Struc: 70 a.a.

Protein chain

P37277  (PSAL_SYNY3) -  Photosystem I reaction center subunit XI from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 157 a.a.

Struc: 137 a.a.

Protein chain

P72986  (PSAM_SYNY3) -  Photosystem I reaction center subunit XII from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 31 a.a.

Struc: 31 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C: E.C.1.97.1.12 - photosystem I.
• Reaction: reduced [plastocyanin] + hnu + oxidized [2Fe-2S]-[ferredoxin] = oxidized [plastocyanin] + reduced [2Fe-2S]-[ferredoxin]

DOI no: 10.3389/fpls.2018.01865

Front Plant Sci 9:1865 (2018)

PubMed id: 30662446

Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803.

S.Y.Netzer-El, I.Caspy, N.Nelson.

ABSTRACT

A single histidine addition to the C-terminus of PsaL of Synechocystis sp. PCC 6803 was previously reported by our lab to shift the trimer-to-monomer ratio of PSI in favor of the monomeric form. P700 re-reduction and NADP⁺ photo-reduction measurements of the PsaL^HIS strain show no effect on PSI activity in comparison to the WT trimeric PSI. Crystal structure of the PsaL^HIS monomeric PSI reveals several alterations that occurred in the trimerisation site of PSI, primarily a deformation of the C-terminus of PsaL and loss of chlorophyll a and β-carotene molecules.