A single histidine addition to the C-terminus of PsaL of Synechocystis
sp. PCC 6803 was previously reported by our lab to shift the trimer-to-monomer
ratio of PSI in favor of the monomeric form. P700 re-reduction and
NADP+ photo-reduction measurements of the PsaLHIS strain
show no effect on PSI activity in comparison to the WT trimeric PSI. Crystal
structure of the PsaLHIS monomeric PSI reveals several alterations
that occurred in the trimerisation site of PSI, primarily a deformation of the
C-terminus of PsaL and loss of chlorophyll a and β-carotene molecules.