PDBsum entry 6ecl

Transferase

PDB id: 6ecl

Contents

Protein chains

556 a.a.

401 a.a.

Ligands

T90

Metals

_MN ×2

Waters ×187

PDB id:

6ecl

Name:

Transferase

Title:

Crystal structure of a 1,2,4-triazole allosteric rnase h inhibitor in complex with HIV reverse transcriptase

Structure:

Reverse transcriptase/ribonuclease h. Chain: a. Fragment: p66 domain. Engineered: yes. Mutation: yes. Reverse transcriptase/ribonuclease h. Chain: b. Fragment: p51 domain. Engineered: yes.

Source:

Human immunodeficiency virus type 1 group m subtype b (isolate bh10). HIV-1. Organism_taxid: 11678. Strain: isolate bh10. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.39Å R-factor: 0.187 R-free: 0.229

Authors:

K.A.Kirby,S.G.Sarafianos

Key ref:

K.A.Kirby and s.g.sarafianos Crystal structure of a 1,2,4-Triazole allosteric rnas inhibitor in complex with HIV reverse transcriptase. To be published, .

Date:

08-Aug-18 Release date: 21-Aug-19

Protein chain

P03366  (POL_HV1B1) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10)

Seq: 1447 a.a.

Struc: 556 a.a.*

Protein chain

P03366  (POL_HV1B1) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10)

Seq: 1447 a.a.

Struc: 401 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.2.7.7.- - ?????
• Enzyme class 2: Chains A, B: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: Chains A, B: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: Chains A, B: E.C.3.1.-.-
• Enzyme class 5: Chains A, B: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: Chains A, B: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 7: Chains A, B: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site