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PDBsum entry 6cod
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PDB id:
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Lyase
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Title:
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Athnl enantioselectivity mutant at-a9-h7 apo y13c,y121l,p126f,l128w, c131t,f179l,a209i with benzaldehyde
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Structure:
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Alpha-hydroxynitrile lyase. Chain: a, b. Fragment: athnl. Synonym: athnl,(r)-hydroxynitrile lyase,(r)-oxynitrilase, methylesterase 5,atmes5. Engineered: yes. Mutation: yes
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Source:
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Arabidopsis thaliana. Mouse-ear cress. Organism_taxid: 3702. Gene: hnl, mes5, at5g10300, f18d22_70. Expressed in: escherichia coli. Expression_system_taxid: 469008.
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Resolution:
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1.80Å
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R-factor:
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0.152
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R-free:
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0.194
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Authors:
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B.J.Jones,R.J.Kazlauskas,R.Desrouleaux
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Key ref:
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B.J.Jones
et al.
Athnl enantioselectivity mutants.
To be published,
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Date:
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12-Mar-18
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Release date:
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20-Mar-19
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PROCHECK
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Headers
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References
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Q9LFT6
(HNL_ARATH) -
Alpha-hydroxynitrile lyase from Arabidopsis thaliana
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Seq:
Struc:
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258 a.a.
259 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 6 residue positions (black
crosses)
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Enzyme class:
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E.C.4.1.2.10
- (R)-mandelonitrile lyase.
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Reaction:
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(R)-mandelonitrile = benzaldehyde + hydrogen cyanide
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(R)-mandelonitrile
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=
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benzaldehyde
Bound ligand (Het Group name = )
corresponds exactly
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+
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hydrogen cyanide
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Cofactor:
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Flavoprotein
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
